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Anti-Methyl-Histone H3 (Me-Lys9)(H3K9me1) antibody, Mouse monoclonal
| 产品编号： | 3376264 |
| 规格： | clone 7E7-H12, purified from hybridoma cell culture |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Methyl-Histone H3 (Me-Lys9)(H3K9me1) antibody, Mouse monoclonal
RP11-396C23.1
H3.3B
H3F3
H3.3A
zgc:86731
H3F3A/H3F3B
zgc:56193
LOC100045490
H3F3A
9430068D06RIK
wu:fb58e10
H3F3B
HISTONE 3B
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | Anti-Methyl-Histone H3 (Me-Lys9) (H3K9me1) antibody, Mouse Monoclonal (mouse IgG1 isotype) is derived from the hybridoma 7E7-H12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a methylated (Me-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH. |
| Immunogen【免疫原】 | Methylated (Me-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH. |
| Application【应用】 | Anti-Methyl-Histone H3 (Me-Lys9) (H3K9me1) antibody has been used in immunoblotting and immunocytochemistry. |
| Biochem/physiol Actions【生化/生理作用】 | Histones are subjected to extensive covalent modifications that play an important role in development and in cancer. These modifications include phosphorylation, methylation, acetylation and ubiquitination. Histones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells. Histone methylation, like acetylation, is a complex, dynamic process involving several processes, including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. Methylation of H3 at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly. |
| Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 7E7-H12, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~17 kDa |
| species reactivity | human |
| concentration【浓度】 | ~1 mg/mL |
| technique(s) | immunoblotting: 2.5-5 μg/mL using human HeLa cells. immunocytochemistry: 2.5-5 μg/mL using human HeLa cells. |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
