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Anti-Mouse IgG (whole molecule)-Agarose antibody produced in goat
产品编号: | 3377442 |
规格: | affinity isolated antibody, suspension |
包装规格: | 2 ML,5 ML,25 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
MDL number | MFCD03791275 |
NACRES | NA.46 |
General description【一般描述】 | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses. |
Immunogen【免疫原】 | IgG isolated from pooled normal mouse serum |
Application【应用】 | Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 ℃. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications. Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) Immunoprecipitation (1 paper) |
Biochem/physiol Actions【生化/生理作用】 | IgG helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG2a of IgG has cytotropic properties and IgG2 molecules have the ability to activate complement. |
Physical form【外形】 | Suspension in 0.5 M NaCl containing preservative |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | goat |
conjugate【偶联物】 | agarose conjugate |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | secondary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | suspension |
species reactivity | mouse |
extent of labeling【标记范围】 | ≥5 mg per mL |
technique(s) | Ouchterlony double diffusion: suitable immunoprecipitation (IP): suitable |
matrix【基质】 | Cross-linked 4% beaded agarose |
matrix activation【基质活化】 | cyanogen bromide |
matrix spacer【基质隔离区】 | 1 atom |
capacity【容量】 | 0.4 mg/mL, resin binding capacity (mouse IgG) |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 2, 5, 25 mL in glass bottle |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 3 |