Anti-NUCB2 (N-terminal) antibody produced in rabbit

NA.44

NUCB2 (N-terminal) (nucleobindin 2, also known as NEFA, p54) belongs to the nucleobindin family of EF-hand Ca²⁺-binding proteins.
Nucleobindin 2 (NUCB2), contain multiple functional domains, including a Leu/Ile-rich region, a putative nuclear localization signal, a DNA binding domain, two Ca²⁺ -binding EF-hand motifs, and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. Human NUCB2 has high homology (62% sequence identity) with human NUCB1, an additional member of the nucleobindin family, although they are encoded by two separate genes.

Anti-NUCB2 (N-terminal) antibody produced in rabbit has been used in:

• immunofluorescence
• western blotting
• immunohistochemistry

Nucleobindin 2 (NUCB2) is implicated in various functions, including Ca²⁺ homeostasis, hypothalamic regulation of feeding, and tumor necrosis factor (TNF)-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and endoplasmic reticulum aminopeptidase 1 (ARTS-1), both through the EF-hand motif. NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been recently identified as a novel satiety molecule in the hypothalamus, associated with melanocortin signaling in the central nervous system. NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~54 kDa

mouse, rat

~1.5 mg/mL

indirect immunofluorescence: 1-2 μg/mL using Rat2 cells
western blot: 1.5-3.0 μg/mL using Rat2 cell lysates
western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction)

P81117

dry ice

−20℃

rat ... Nucb2(59295)

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12 - Non Combustible Liquids

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