抗 周脂素 A 兔抗

MFCD07370428

NA.41

Perilipin is an intracellular neutral lipid storage droplet surface protein in white and brown fat adipocytes. It is also found in lower quantity coating droplets in steroidogenic-cells of the adrenal cortex, ovaries and testicular Leydig cells, on the surface of smaller droplets containing cholesteryl esters. Perilipin has multiple isoforms, resulting from differential splicing events. Perilipin A is most abundant in adipocytes and steroidogenic cells. Perilipin B is a minor form in adipocytes. Steroidogenic cells selectively express perilipins C and D.

synthetic peptide corresponding to amino acid residues 492-505 of human perilipin A with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one residue in mouse and rat.

Anti-Perilipin A antibody produced in rabbit has also been used in:

• indirect immunofluorescence
• immunoblotting
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Perilipin is a gatekeeper protein that is involved in regulating triacylglycerol storage in adipocyte through the suppression of basal lipolysis apparently through protecting triacylglycerol against hydrolysis. Perilipin also enhances cyclic adenosine monophosphate (c-AMP)-dependent protein kinase (PKA)-stimulated lipolysis by hormone-sensitive lipase (HSL) and non-HSLs. Perilipin knockout mice exhibit reduced adipose tissue mass and resistance to diet induced obesity. Their lipid storage droplets are coated with adipose differentiation-related protein (ADRP, adipophilin), which is not phosphorylated by PKA.

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~62 kDa

human, mouse

indirect immunofluorescence: 5-10 μg/mL using differentiated mouse NIH3T3-L1 cells.
western blot (chemiluminescent): 1-2 μg/mL using whole extract of differentiated mouse NIH3T3-L1 cells

dry ice

−20℃

human ... PLIN(5346)
mouse ... Plin(103968)
rat ... Plin(25629)

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10 - Combustible liquids

WGK 3