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Anti-phospho-PKR (pThr451) antibody produced in rabbit

产品编号:3385533
规格:affinity isolated antibody, buffered aqueous glycerol solution
包装规格:1 VIAL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
33855331 VIAL4910
产品别名

Anti-phospho-PKR (pThr451) antibody produced in rabbit

Anti-phospho-Interferon-Inducible Double-Stranded RNA-Dependent Protein (pThr451)

基本信息
MDL number
MFCD03455798
NACRES
NA.44
General description【一般描述】
Protein kinase regulated by RNA (PKR) is an interferon-inducible ser/thr protein kinase, activated by stress signals and double-stranded RNA (dsRNA). In response to viral infection, PKR is activated by dsRNA-mediated dimerization and autophosphorylation. α-subunit of protein synthesis initiation factor 2 (eIF-2α) is well-studied substrate of PKR. Activation by PKR leads to phosphorylation on Ser51 that result in inhibition of translation. Human PKR contains at least 15 autophosphorylation sites. But phosphorylation on threonine 446 and 451 in the PKR activation loop is required in vivo and in vitro for high-level kinase activity. PKR is implicated in signalling pathways that involve NF-κB, p38, and IRF-3 and is the key effector in IFN-induced anti-viral responses, apoptosis and protein synthesis.
Anti-Phospho-PKR [pThr451] specifically recognizes PKR phosphorylated on threonine 451 (65-68 kDa). The antibody detects human and mouse PKR [pThr451].
Immunogen【免疫原】
synthetic phosphopeptide derived from the region of PKR that is phosphorylated on threonine 451.
Application【应用】
Anti-phospho-PKR (pThr451) antibody may be used for detection by immunoblotting at a working dilution of 1:1,000 using human HeLa cells stimulated with IFN·γ and calyculin. For detection by immunoblotting in human HeLa cells, a working concentration of 0.1-1.0 μg/mL is recommended. Detection of phospho-PKR (pThr451) was done in peritoneal macrophages obtained from thioglycolate-treated mice and human peripheral blood mononuclear cells at a working dilution of 1:500
Physical form【外形】
Supplied as a solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide. The amount of the reagent is sufficient for 10 blots.
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
200
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous glycerol solution
mol wt【分子量】
antigen 65-68 kDa
species reactivity
human, mouse
technique(s)
immunoblotting: suitable using human HeLa cells stimulated with IFNγ and calyculin
UniProt accession no.【UniProt登记号】
P19525
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
Gene Information
human ... EIF2AK2(5610)
mouse ... Eif2ak2(19106)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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