广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-POU5F1 (Oct4) antibody produced in rabbit
产品搜索:

Anti-POU5F1 (Oct4) antibody produced in rabbit

产品编号:3387527
规格:~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3387527200 μL5040
产品别名

Anti-POU5F1 (Oct4) antibody produced in rabbit

Anti-OTF3

Anti-Octamer-binding transcription factor 3

Anti-OCT4

Anti-OTF4

Anti-OCT3

Anti-POU domain, class 5, transcription factor 1

产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
200
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen ~43 kDa
species reactivity
human
concentration【浓度】
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using NT2 cell lysate
indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells
western blot: 1-2 μg/mL using NT2 cell lysate
UniProt accession no.【UniProt登记号】
Q01860
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)
基本信息
General description【一般描述】
POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain.
POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33.
Immunogen【免疫原】
synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous.
Application【应用】
Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting.
Biochem/physiol Actions【生化/生理作用】
POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma.
POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process.
Physical form【外形】
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0094