Anti-PTPRF (C-terminal) antibody produced in rabbit

NA.44

PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule.
Protein tyrosine phosphatase, receptor type F (PTPRF) consists of noncovalently bound subunits designated as E (extracellular) and P (phosphatase) subunits that are derived from a precursor protein by proteolytic processing. The E subunit (150 kDa) has three immunoglobulin-like domains and eight fibronectin III-like domains, and the P subunit (85 kDa) has a short extracellular domain, a transmembrane segment and tandem cytoplasmic PTP catalytic domains.

The antibody recognizes both the extracellular and the cytoplasmic subunits of human PTPRF. Anti-PTPRF (C-terminal) antibody is specific for the P- subunit and the whole PTPRF protein from Human origin. Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.

The antibody may be used in several immunochemical techniques including immunoblotting (∼210, ∼150, and ∼85 kDa) and immunoprecipitation.
Anti-PTPRF (C-terminal) antibody produced in rabbit has been used in an enzyme based assay of protein tyrosine phosphatase 1 (PTP1).

Protein tyrosine phosphatase, receptor type F (PTPRF) removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function. Insulin receptor, epidermal growth factor receptor (EGF) receptor, hepatocyte growth factor (HGF) receptor and rearranged during transfection (RET) have been reported as substrates of PTPRF.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous solution

antigen ~150 kDa
antigen ~210 kDa
antigen ~85 kDa

human

immunoprecipitation (IP): 5-10 μL using lysates of HEK-293T cells over-expressing human PTPRF.
western blot: 1:500-1:1000 using lysates of HEK-293T cells over-expressing human PTPRF.

P10586

dry ice

−20℃

human ... PTPRF(5792)
mouse ... Ptprf(19268)
rat ... Ptprf(360406)

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12 - Non Combustible Liquids