Anti-Rab18 antibody produced in rabbit

NA.41

Rab18 is a member of the Rab family of small guanosine triphosphatases (GTPases). The Rab family belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif.

Anti-Rab18 antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Rab guanosine triphosphatases (Rab GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and GTP-bound active states. Activation of a Rab protein is coupled to its association with intracellular membranes. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement and membrane fusion. The conserved regions of Rab proteins are implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. Therefore, antibodies to Rab proteins may serve as useful tool for studying subcellular localization and membrane organization. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

~23 kDa (an additional band at ∼43 kDa may appear in some extract preparations)

rat, human, mouse

~1.0 mg/mL

immunoprecipitation (IP): 5-10 μg using lysates of human PC3 cells
indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells
western blot: 1-2 μg/mL using whole extracts of mouse MM3MG cells

Q9NP72

dry ice

−20℃

human ... RAB18(22931)
mouse ... Rab18(19330)
rat ... Rab18(307039)

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12 - Non Combustible Liquids