Anti-Rho-GDI antibody produced in rabbit

MFCD01634148

NA.41

Rho guanine nucleotide dissociation inhibitor I (RhoGDI), also known as Rho GDP dissociation inhibitor α (ARHGDIA) is encoded by the gene mapped to human chromosome 17q25.3-25-3. RhoGDI is the member of Rho/Rac protein family. The encoded protein consists of 204 amino acids and has a molecular mass of 23,421,000 Mr. In mammals, three RhoGDI isoforms exists: RhoGDIα, RhoGDIβ and RhoGDIγ. RhoGDIα is widely expressed. RhoGDIβ is mainly expressed in hematopoietic cells and RhoGDIγ is present in the brain, lung, kidney and testis.
Rho proteins are basically described as guanine nucleotide-binding proteins (GNBPs). RhoGDI (Rho guanine nucleotide dissociation inhibitor)/ARHGDIA (Rho guanine nucleotide dissociation inhibitor) has three subtypes namely- RhoGDIα (Rho GDP dissociation inhibitor α), RhoGDIβ (Rho GDP dissociation inhibitor β) and RhoGDIγ (Rho GDP dissociation inhibitor γ). RhoGDIα is expressed everywhere. It is located on human chromosome 17q25.3. Its protein has 204 amino acids and belongs to the Rho/Rac family.

The antibody recognizes Rho-GDI and cross-reacts with D4-GDI.

GST fusion protein corresponding to full-length human Rho-GDI.

Anti-Rho-GDI has been used in western blotting.
Anti-Rho-GDI antibody produced in rabbit has been used in immunoblotting.

Rho proteins control the actin and microtubule cytoskeleton. RhoGDIα is necessary for the spatial and temporal modulation of RhoGNBPs (guanine nucleotide-binding proteins). In HCC (hepatocellular carcinoma) patients, absence of ARHGDIA (Rho guanine nucleotide dissociation inhibitor) affects the prognosis and induces invasion and metastasis of HCC cells.

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

Purified using protein A

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous solution

antigen ~28 kDa

bovine, human, mouse

~1 mg/mL

immunoprecipitation (IP): 4 μg using 500 μg of Jurkat RIPA lysate
western blot: 0.5-1.0 μg/mL using RIPA lysates from human HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells and mouse 3T3/A31

P52565

dry ice

−20℃

human ... ARHGDIA(396)
mouse ... Arhgdia(192662)

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10 - Combustible liquids

WGK 1