Anti-SERPINA3 antibody produced in rabbit

MFCD00162119

HPA000893    Human Protein Atlas characterization data

The α1-antichymotrypsin (ACT) protein has a molar mass of 55-66kDa. It has a serpin structure with three β sheets, 8 α helices and a reactive centre loop containing the active site. The gene is mapped to human chromosome 14q32.1.

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

α1-antichymotrypsin (ACT) is an α-globulin glycoprotein that is a member of the serpin superfamily and is encoded by the SERPINA3 gene in humans. Its expression is higher in prostate cancer than in benign prostatic hyperplasia. The serine protease has been found to be associated with several diseases such as chronic obstructive pulmonary disease (COPD), Parkinson′s disease (PD), Alzheimer′s disease (AD), stroke, cystic fibrosis, cerebral haemorrhage and multiple system atrophy. The encoded protein forms a complex with toxic β-amyloid (Aβ). The gene expression is induced by cytokines. ACT inhibits serine proteases, such as pancreatic chymotrypsin, leukocyte cathepsin G, mast cell chymases, human glandular kallikrein 2, kallikrein 3, and pancreatic cationic elastase. The levels of circulating ACT increases with inflammation. It also has the ability to bind to DNA unlike other serpins.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST82412.

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

independent
Learn more about Antibody Enhanced Validation

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:2500-1:5000

LASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGGAPHR

P01011

wet ice

−20℃

human ... SERPINA3(12)

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10 - Combustible liquids

WGK 1