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Cholesterol Oxidase microbial
产品价格查看更多规格...
| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 3425536 | 100 UNITS | 3140 |
产品别名
9028-76-6
Cholesterol Oxidase microbial
Cholesterol: oxygen oxidoreductase
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 1.1.3.6 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-842-1 |
| MDL number | MFCD00130783 |
| NACRES | NA.54 |
| Application【应用】 | Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures. |
| Biochem/physiol Actions【生化/生理作用】 | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity. |
| Unit Definition【单位定义】 | One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 ℃ and pH 7.0 in a peroxidase linked system. |
| Preparation Note【制备说明】 | Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use. |
产品性质
| Quality Level【质量水平】 | 200 |
| form【形式】 | lyophilized powder |
| Specific Activity | ≥10 units/mg protein |
| mol wt【分子量】 | 55 kDa |
| solubility【溶解性】 | 50 mM potassium phosphate buffer, pH 7.0: soluble |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 units in glass bottle |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
