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Clostripain from Clostridium histolyticum
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| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 3426750 | 250 UNITS | 9420 |
产品别名
9028-00-6
Clostripain from Clostridium histolyticum
Proteinase from Clostridium histolyticum
Clostridiopeptidase B
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.22.8 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-822-2 |
| MDL number | MFCD00130814 |
| NACRES | NA.54 |
| Application【应用】 | Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43). |
| Biochem/physiol Actions【生化/生理作用】 | Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds. |
| Quality【质量】 | Purified, essentially salt-free |
| Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 ℃ in the presence of 2.5 mM DTT. |
| Analysis Note【分析说明】 | The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate. |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 100 |
| Specific Activity | ≥20 units/mg solid |
| mol wt【分子量】 | 15.4 kDa 41.7 kDa |
| impurities【杂质】 | salt, essentially free |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | Bottomless glass bottle. Contents are inside inserted fused cone. |
安全信息
| Pictograms【象形图】 | GHS07,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H319 - H334 - H335 |
| Precautionary Statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
