您的位置:首页 > 产品中心 > CypExpressTM 1A2 Cytochrome P450 human
CypExpressTM 1A2 Cytochrome P450 human
产品别名
CypExpressTM 1A2 Cytochrome P450 human
CP12
P450(PA)
CYP1A2
P450 1A2
P3-450
基本信息
EC number【EC 号】 | 232-387-9 |
NACRES | NA.77 |
Application【应用】 | Poster: A Novel, Robust Recombinant Human P450 Biocatalytic System Poster: Evaluation of Dry Powder Preparations of Permeabilized Pichia pastoris Cells Expressing Human Cytochrome P450 2D6 Enzyme (CYP2D6) for Drug Metabolite Generation Poster: Facile Scale-up Biocatalytic Production of P-450 Metabolites Using a Novel Recombinant Human Catalytic System Poster: Multi-Cycle Production of Metabolites with human P450 systems Poster: Rapid identifcation and production of metabolites using CypExpress™ 2D6m 3A4 and 2C9 Poster: Rapid identifcation and production of metabolites using stabilized dried powder (SDP) of human cytochrome P450S (hCYPS) 2D6, 3A4, and 2C9 engineered in yeast Poster: Utilizing a Novel Biocatalytic Human P450 System for the Multi - Cycle Production of Metabolites |
Biochem/physiol Actions【生化/生理作用】 | Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Furafylline and α-naphthoflavone are inhibitors, whereas omeprazole and lansoprazole are inducers of the enzyme. The isozyme CYP1A2 is a major pathway for the in vivo conversion of phenacetin into acetaminophen via O-deethylation. This isozyme also catalyzes the 5-hydroxylation of thiabendazole. Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. |
Features and Benefits【特点和优势】 | CypExpress™ 1A2 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 1A2 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress™ are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress™ retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation. |
Unit Definition【单位定义】 | A reaction mixture containing 500 μM phenacetin and 100 mg/mL of CypExpress™ 1A2 will produce 35 μM acetaminophen in 4 hours at 30℃ under the conditions described. |
Physical form【外形】 | Light yellow, very fine powder |
Reconstitution【重悬】 | Per Protocol/Variable |
Legal Information【法律信息】 | 6538 is a trademark of American Type Culture Collection CypExpress is a trademark of Oxford Biomedical Research |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in Pichia pastoris |
form【形式】 | dry powder |
UniProt accession no.【UniProt登记号】 | P05177 |
application(s) | cell analysis |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... CYP1A2(1544) |
安全信息
Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
WGK | WGK 3 |