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Bolton Broth Selective Supplement

产品编号:4060821
规格:according to ISO 10272-1, for Campylobacter spp., pkg of 10 vials, For the preparation of Bolton Selective Enrichment Broth
包装规格:10 VIAL
产品类别:进口试剂
品牌:Millipore
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
406082110 VIAL4160
产品别名

Bolton Broth Selective Supplement

Campylobacter Selective Supplement

产品性质
Quality Level【质量水平】
200
agency
according to ISO 10272-1
reg. compliance
FDA -BAM
form【形式】
lyophilized powder
composition【组成】
Amphotericin B, 5 mg
Cefoperazone, 10 mg
Trimethoprim, 10 mg
Vancomycin, 10 mg
packaging【包装】
pkg of 10 vials
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
pathogen testing
storage temp.【储存温度】
2-8℃
suitability【适用性】
Campylobacter spp.
Listeria spp.
基本信息
General description【一般描述】
Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.
Application【应用】
Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.
Linkage【联系】
Replaces: 1.00079.0017
Analysis Note【分析说明】
Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004):
10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004):
>10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023): no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004):
greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156): greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic
安全信息
Pictograms【象形图】
GHS08
Signal word【警示用语:】
Danger
Hazard Statements
H317 - H334 - H361d - H373
Precautionary Statements
P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
Hazard Classifications【危险分类】
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 3
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