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Bcl-xL BH44-23, Human, Cell-Permeable-Calbiochem
| 产品编号： | 4061643 |
| 规格： | A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. |
| 包装规格： | 1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Bcl-xL BH44-23, Human, Cell-Permeable-Calbiochem
HIV-TAT48-57-β-Ala-Bcl-x L BH44-23, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
基本信息
| Empirical Formula【经验(实验)分子式】 | C165H279N59O46 |
| Molecular weight | 3825.35 |
| General description【一般描述】 | A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-xL (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria. A highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-xL (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria. |
| Biochem/physiol Actions【生化/生理作用】 | Primary Target Voltage-dependent anion channel (VDAC) Product does not compete with ATP. Cell permeable: yes Reversible: no |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Sequence【序列】 | Ac-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH |
| Physical form【外形】 | Supplied as a trifluoroacetate salt. |
| Reconstitution【重悬】 | Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. |
| Other Notes【其他说明】 | Chen m., et al. 2002. J. Biol. Chem. 277, 29181. Futaki, S., et al. 2001. J. Biol. Chem.276, 5836. Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA97, 3100. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| Assay【测定】 | ≥95% (HPLC) |
| form【形式】 | lyophilized |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze desiccated (hygroscopic) |
| solubility【溶解性】 | 20% acetic acid: 5 mg/mL DMSO: 5 mg/mL |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 1 mg in Plastic ampoule Packaged under inert gas |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
