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ERK Activation Inhibitor Peptide I, Cell-Permeable-Calbiochem
| 产品编号： | 4061671 |
| 规格： | The ERK Activation Inhibitor Peptide I, Cell-Permeable controls the biological activity of ERK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications. |
| 包装规格： | 1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
ERK Activation Inhibitor Peptide I, Cell-Permeable-Calbiochem
Ste-MEK113, Ste-MPKKKPTPIQLNP-NH2, Erk Inhibitor IV
基本信息
| Empirical Formula【经验(实验)分子式】 | C86H153N19O17S |
| Molecular weight | 1757.32 |
| General description【一般描述】 | A cell-permeable, reversible, and stearated, 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK). Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 2.5 µM). Shown to block ERK activity in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC50 = 13 µM). Does not affect the activation of either JNKs or p38 MAP kinase. A cell-permeable and reversible stearated 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 2.5 µM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC50 = 13 µM). Does not affect the activation of JNKs or p38. |
| Biochem/physiol Actions【生化/生理作用】 | Primary Target MEK Product does not compete with ATP. Reversible: yes Cell permeable: yes |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Sequence【序列】 | Ste-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated) |
| Physical form【外形】 | Supplied as a trifluoroacetate salt. |
| Reconstitution【重悬】 | Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. |
| Other Notes【其他说明】 | Kelemen, B.R., et al. 2002. J. Biol. Chem.277, 8741. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| Assay【测定】 | ≥95% (HPLC) |
| form【形式】 | lyophilized solid |
| potency【效能】 | 2.5 μM IC50 |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze desiccated (hygroscopic) protect from light |
| color【颜色】 | white |
| solubility【溶解性】 | water: 1 mg/mL DMSO: 5 mg/mL |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 1 mg in Plastic ampoule Packaged under inert gas |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
