ATP Assay Kit

sufficient for 200 tests

100

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

sample type sludge
sample type blood
sample type milk
sample type soil
sample type urine

chemiluminescent

−70℃

1 kit in Glass bottle

Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in the cell has been used as an indicator of cell death. In contrast, increased levels of ATP has been an indicator of cell proliferation. Calbiochem^® brand ATP Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter.
The assay is based on the firefly luciferase-catalyzed oxidation of D-luciferin in the presence of ATP and oxygen, whereby the amount of ATP is quantified by the amount of light (hv) produced.

One vial of Luciferase ATP Monitoring Enzyme, Enzyme Reconstitution Buffer, 1 bottle of Nucleotide Releasing Reagent, one vial of ATP Standard, and a user protocol.

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Assay Time: 1 h

The assay can be fully automatic for high throughput (10 s/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.

Reagent Reconstitution1. Reconstitute ATP Monitoring Enzyme with 2ml of the Enzyme Reconstitution Buffer. Mix well by gentle pipetting. The reconstituted enzyme is stable for up to 2 months at 4°C.2. Prepare an ATP standard solution by dissolving the 1 mg ATP into 1 ml of H₂O. The solution is stable for several weeks at -20°C.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Upon arrival store the entire contents of the kit at -70°C.

GHS05,GHS07,GHS08,GHS09

Danger

H302 - H315 - H318 - H373 - H410

P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P314

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral

Kidney

10 - Combustible liquids

WGK 3