Calmodulin Kinase IINtide, Myristoylated-Calbiochem

C₂₅₆H₂₇₅N₄₇O₄₃

4698.21

The myristoylated form of CaMK IINtide (Cat. No. 208920). The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.
The myristoylated form of CaMK IINtide (Cat. No. 208920), a potent, specific inhibitor of Ca²⁺/Calmodulin kinase II (CaMK II) (IC₅₀ = 50 nM for total and Ca²⁺-independent CaMK II activity). The peptide sequence corresponds to the inhibitory domain of the CaMK II inhibitory protein, CaMK IIN. Exhibits inhibitory activity across converged species, including rat brain, goldfish brain, and Drosophila (IC₅₀ = 100-400 nM). Shown to completely inhibit the phosphorylation of GluR1 fusion protein at a concentration of 1 µM. Does not inhibit CaMK I, CaMK IV, CaMKK, PKA, or PKC. The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

Product does not compete with ATP.
Target IC₅₀: 50 nM against cam Kinase-2
Cell permeable: yes
Primary Target
Calmodulin-Dependent Protein Kinase (CaM Kinase)-2
Reversible: no

Toxicity: Standard Handling (A)

Myr-N-Gly-Gly-Gly-Lys-Arg-Pro-Pro-Lys-Leu-Gly-Gln-Ile-Gly-Arg-Ala-Lys-Arg-Val-Val-Ile-Glu-Asp-Asp-Arg-Ile-Asp-Asp-Val-Leu-Lys-OH

Supplied as a trifluoroacetate salt.

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at-20°C.

Sodering, T.R., et al. 2001. J. Biol. Chem.276, 3719.
Chang, B.H., et al. 1998. Proc. Natl. Acad. Sci. USA95, 10890.
Pereda, A.E., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13272.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
desiccated (hygroscopic)

white

DMSO: 5 mg/mL

ambient

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1