DAR-4M-Calbiochem

C₂₅H₂₆N₄O₃

430.50

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

Primary Target
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Irritant (B)

In DMSO.

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solution

Calbiochem^®

OK to freeze
protect from light

red-violet

λ_ex ~560  nm
λ_em ~575 nm

ambient

−20℃

1 mg in Plastic ampoule

10 - Combustible liquids

WGK 2