Ubiquitin, Methylated, Human, Recombinant

≥95% (SDS-PAGE)

100

lyophilized solid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

aqueous buffer: 50 mg/mL

wet ice

−20℃

1 mg in Plastic ampoule

Recombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1