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产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human cervix |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 46 |
| morphology【形态学】 | Epithelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human cervix carcinoma |
| Cell Line Description【细胞系描述】 | Derived from the parent HeLa line but differs chiefly in its relative insusceptibility to polioviruses. The presence of human papilloma virus 18 (HPV-18) sequences in HeLa cells has been reported. |
| Application【应用】 | Tumourigenicity and virus studies, poliovirus 1 and AD3 |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 9,10 D13S317: 14 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Culture Medium【培养基】 | EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
