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产品性质
| biological source【生物来源】 | hamster ovary |
| description【描述】 | CHO-K1, FcγRIIIa, Fc gamma receptor 3A, 158F, allotype, human IgG, IgG binding |
| growth mode【生长模式】 | Adherent |
| morphology【形态学】 | Polygonal |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Stably transfected with a pcDNA3.1/Hygro(+) based vector with a human Fc?RIIIa 158F cDNA insert that had been modified to enable expression as a GPI linked receptor |
| Cell Line Description【细胞系描述】 | Clone of CHO-K1 cells expressing human FcγRIIIa of the 158F allotype anchored to glycosyl phosphatidylinositol |
| Culture Medium【培养基】 | MEM (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 10% FBS (F2442) with 600ug/ml hygromycin B |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO2/95%air; 37℃ |
| Other Notes【其他说明】 | Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
| Citation【引用】 | If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.CI7 (ECACC 15042902) |
产品说明
| Disclaimer【免责声明】 | This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. |
