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产品别名
UKKi007-A
EBiSC iPSC Line
iPSC
Human iPSC
iPS Cell
Induced Pluripotent Stem Cell Line
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human dermis (fibroblast) |
| reprogramming method【重编程方法】 | retrovirus |
| description【描述】 | age (45-49) |
| manufacturer/tradename | EBiSC™ |
| gender【性别】 | female |
| growth mode【生长模式】 | adherent (pluripotent) |
| technique(s) | cell culture | stem cell: suitable |
| relevant disease(s)【相关疾病】 | ventricular tachycardia |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| General description【一般描述】 | Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop. The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching. |
| Cell Line Origin【细胞系来源】 | Depositor Klinikum der Universität zu Köln |
| Cell Line Description【细胞系描述】 | Derivation Primary cell type: Fibroblast of dermis Primary cell developmental stage: Adult Location of primary tissue procurement: Berlin, Germany Tissue collection date: June 15, 2009 Passage number reprogrammed: P3 Reprogramming method Vector type: Integrating Vector: Virus Virus type: Retrovirus Have the reprogramming vectors been silenced: Yes Merthods used: rtpcr Notes on reprogramming vector silencing: Retrovirally encoded reprogramming factors OCT4, KLF4 and cMYC are not expressed as determined by RT-PCR. Only Sox2 seems to be expressed. at very low levels. Files showing reprogramming vector expressed or silenced: Image / Result Vector map: File Characterization Analysis of Undifferentiated Cells Marker expression: TRA 1-80(+)SSEA-4 (+)POU5F1 (OCT-4) (+)Alkaline Phosphatase(+)NANOG(+) Differentiation potency Ectoderm: In vivo teratoma Endoderm: In vivo teratoma Mesoderm: In vivo teratoma In vitro spontaneous differentiation Microbiology / Virus Screening HIV 1: Negative HIV 2: Not done Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation Genotyping Karyotyping Passage number: 34 Cell line karyotype: 46, XX Karyotyping method: Molecular karyotyping by SNP array Genotyping Genome-wide analysis: SNP genotyping using OmniExpress Exome Chip STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase. |
| Linkage【联系】 |
Note: EAUA and CLIP must be completed before order fulfillment |
| Subculture Routine【传代培养常规】 | Medium: Essential E8® Passage method: EDTA Matrix: Vitronectin CO2 concentration: 5% O2 concentration: 21% Temperature: 37℃ |
| Legal Information【法律信息】 | E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation EBiSC is a trademark of Fraunhofer-Gesellschaft |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
