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产品性质
| biological source【生物来源】 | human peripheral blood |
| reprogramming method【重编程方法】 | episomal |
| description【描述】 | age (30-34) |
| manufacturer/tradename | EBiSC™ |
| gender【性别】 | male |
| growth mode【生长模式】 | adherent (pluripotent) |
| technique(s) | cell culture | stem cell: suitable |
| relevant disease(s)【相关疾病】 | none |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| General description【一般描述】 | Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop. The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching. |
| Cell Line Origin【细胞系来源】 | Depositor Klinikum der Universität zu Köln |
| Cell Line Description【细胞系描述】 | Derivation Primary cell type: Peripheral blood mononuclear cell Tissue collection year: 2016 Passage number reprogrammed: 3 Reprogramming method Vector type: Non-integrating Vector: Episomal Is the reprogramming vector detectable: No Merthods used: pcr Notes on reprogramming vector detection: episomal vector cassette absence Files showing reprogramming vector expressed or silenced: Image / Result Xeno free conditions: no Derived under gmp: no Available as clinical grade: no Characterization Analysis of Undifferentiated Cells Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles CD90 Yes - FOXD3 Yes - NANOG Yes - - OCT 4 Yes - - SOX2 Yes - SSEA-4 Yes - - TRA 1-80 Yes - - SSEA-1 Yes - DNMT3b Yes - Morphology: morphology pictures of iPS cell line NP0100-8 before banking and of the thawing control morphology pictures of iPS cell line NP0100-8 before banking and of the thawing control Differentiation potency Ectoderm: Ectoderm In vitro directed differentiation Marker Expressed Nestin Unknown Morphology: expression of NESTIN in differentiated iPS cell line NP0100-8 Endoderm: Endoderm In vitro directed differentiation Marker Expressed SOX17 Yes Morphology: expression of SOX17 in differentiated iPS cell line NP0100-8 Mesoderm: Mesoderm In vitro directed differentiation Marker Expressed BRACHYURY Yes Morphology: expression of BRACHYURY in differentiated iPS cell line NP0100-8 Microbiology / Virus Screening HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Genotyping Karyotyping Passage number: 26 Cell line karyotype: No larger chromosomal aberrations observed Karyotyping method: Molecular karyotyping by SNP array Image / Result |
| Linkage【联系】 |
Note: EAUA and CLIP must be completed before order fulfillment |
| Subculture Routine【传代培养常规】 | Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: Essential E8® Passage Number banked (pre-EBiSC): 26 No. Vials banked (pre-EBiSC): 25 |
| Legal Information【法律信息】 | E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation EBiSC is a trademark of Fraunhofer-Gesellschaft |
安全信息
| Storage Class Code【储存分类代码】 | 6.2 - Infectious substances |
| WGK | WGK 3 |
