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Anti-phospho-TrkA (pTyr490) antibody produced in rabbit
| 产品编号： | 4063525 |
| 规格： | affinity isolated antibody, buffered aqueous glycerol solution |
| 包装规格： | 0.1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD01092507 |
| NACRES | NA.41 |
| General description【一般描述】 | TrkA belongs to the Trk proto-oncogene family and is expressed mainly in central and peripheral nervous systems. When TrkA binds to NGF, it autophosphorylates tyrosine residues like-Tyr490, Tyr674, Tyr675, Tyr751, and Tyr785 and subsequently activates many downstream effector proteins. Defects in TrkA lead to congenital insensitivity to pain with anhidrosis. Anti-phospho-TrkA (pTyr490) antibody can be used in immunocytochemistry (1:100, using cultured cells). It is also useful in identifying endogenous levels of phosphorylated Tyr490 in TrkA, TrkB and TrkC. Rabbit anti- phospho-TrkA (pTyr490) antibody is reactive in mouse, human and rat. |
| Immunogen【免疫原】 | synthetic phosphopeptide Tyr490 corresponding to residues surrounding Tyr490 of human TrkA, conjugated to KLH. This sequence is highly conserved in TrkB and TrkC. |
| Application【应用】 | Anti-phospho-TrkA (pTyr490) antibody can be used in immunoprecipitation, immunoblotting and immunohistochemistry. It may also be used in western blotting when diluted to ratio of 1:1000 for NGF-treated PC12 cells. |
| Physical form【外形】 | Solution in 10 mM sodium HEPES, pH 7.5, and 150 mM sodium chloride containing 100 μg/ml bovine serum albumin and 50% glycerol. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous glycerol solution |
| mol wt【分子量】 | antigen 140 kDa |
| species reactivity | human, mouse, rat |
| technique(s) | immunocytochemistry: 1:100 using cultured cells immunohistochemistry (frozen sections): 1:10 immunoprecipitation (IP): 1:250 western blot: 1:1,000 using NGF-treated PC12 cells |
| UniProt accession no.【UniProt登记号】 | P04629 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... NTRK1(4914) mouse ... Ntrk1(18211) rat ... Ntrk1(59109) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
