Auto-Activated Protein Kinase from bovine kidney

MFCD03792079

NA.32

This enzyme phosphorylates and inactivates protein phosphatase 2A. The funtion appears to be related to the catalytic domain of p21-activated p65^(PAK) protein kinase which is produced in apoptotic cells. AK is also involved in cytoskeleton organization and other signal transduction processes.

One unit is the amount of enzyme that will incorporate 1 nmole of phosphoryl groups using myelin basic protein as a substrate per minute at pH 7 at 30 deg C.

50 ng per vial in 10 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 65% ethyleneglycol, and 0.1% Brij^® 35.

Brij is a registered trademark of Croda International PLC

200

bovine kidney

Minimum 50 ng protein/vial by Bradford

aqueous solution

~17,000 units/mg protein

36 kDa

dry ice

−70℃