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Cathepsin D/E Substrate, Fluorogenic-Calbiochem

产品编号:4063724
规格:An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L .
包装规格:1 MG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40637241 MG4180
产品别名

Cathepsin D/E Substrate, Fluorogenic-Calbiochem

MOCAc-GKPILF~FRLK(Dnp)-D-R-NH2

MOCAc-GKPILF~FRLK(Dnp)- D-R-NH2

基本信息
Empirical Formula【经验(实验)分子式】
C85H122N22O19
Molecular weight
1756.01
General description【一般描述】
An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
Biochem/physiol Actions【生化/生理作用】
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Substrate for cathepsins D and E
kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
Warning【警告】
Toxicity: Standard Handling (A)
Sequence【序列】
MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂

MOCAc = (7-Methoxycoumarin-4-yl)acetyl
Physical form【外形】
Supplied as a trifluoroacetate salt.
Reconstitution【重悬】
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes【其他说明】
Yasuda, Y., et al. 1999. J. Biochem.126, 260.
Yasuda, Y., et al. 1999. J. Biochem.125, 1137.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
Assay【测定】
≥97% (HPLC)
form【形式】
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
desiccated (hygroscopic)
protect from light
color【颜色】
yellow
solubility【溶解性】
DMSO: 5 mg/mL
fluorescence【荧光】
λex ~328  nm
λem ~393 nm
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
packaging【包装】
1 mg in Plastic ampoule
Packaged under inert gas
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