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Cathepsin D/E Substrate, Fluorogenic-Calbiochem
产品编号: | 4063724 |
规格: | An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L . |
包装规格: | 1 MG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Cathepsin D/E Substrate, Fluorogenic-Calbiochem
MOCAc-GKPILF~FRLK(Dnp)-D-R-NH2
MOCAc-GKPILF~FRLK(Dnp)- D-R-NH2
基本信息
Empirical Formula【经验(实验)分子式】 | C85H122N22O19 |
Molecular weight | 1756.01 |
General description【一般描述】 | An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates. An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm. |
Biochem/physiol Actions【生化/生理作用】 | Product does not compete with ATP. Reversible: no Cell permeable: no Primary Target Substrate for cathepsins D and E kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively |
Warning【警告】 | Toxicity: Standard Handling (A) |
Sequence【序列】 | MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂ MOCAc = (7-Methoxycoumarin-4-yl)acetyl |
Physical form【外形】 | Supplied as a trifluoroacetate salt. |
Reconstitution【重悬】 | Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. |
Other Notes【其他说明】 | Yasuda, Y., et al. 1999. J. Biochem.126, 260. Yasuda, Y., et al. 1999. J. Biochem.125, 1137. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
Assay【测定】 | ≥97% (HPLC) |
form【形式】 | lyophilized solid |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze desiccated (hygroscopic) protect from light |
color【颜色】 | yellow |
solubility【溶解性】 | DMSO: 5 mg/mL |
fluorescence【荧光】 | λex ~328 nm λem ~393 nm |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 1 mg in Plastic ampoule Packaged under inert gas |