MTT-CAS 298-93-1-Calbiochem

C₁₈H₁₆BrN₅S

414.32

MFCD00011964

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (silver nitrate titration)

solid

Calbiochem^®

OK to freeze

≤0.5% Sulfated ash

yellow

PBS: 2 mg/mL

heavy metals: ≤0.001%

ambient

15-25℃

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

AZKSAVLVSZKNRD-UHFFFAOYSA-M

1 g in Glass bottle
10 g in Plastic ampoule

GHS07,GHS08

Warning

H315 - H319 - H335 - H341

P201 - P202 - P261 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Respiratory system

11 - Combustible Solids

WGK 3