您的位置：首页 > 产品中心 > 5′-Nucleotidase (CD73) Activity Assay Kit (Colorimetric)
5′-Nucleotidase (CD73) Activity Assay Kit (Colorimetric)
产品性质
| Quality Level【质量水平】 | 100 |
| web metadata keyword.default | 5′-Nucleotidase (CD73) Activity Assay Kit (Colorimetric)|sigma-aldrich |
| Detection Method | colorimetric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | The kit is suitable for the measurement of 5′-Nucleotidase activity in animal tissue lysates (eg. liver), cell lysates, recombinant enzyme and purified protein. |
基本信息
| General description【一般描述】 | 5′-Nucleotidase (5′-NT), also known at CD73 is an enzyme located in the plasma membrane. It converts extracellular nucleotides like 5′-AMP to their corresponding nucleosides, through phosphorylitic cleavage. This conversion facilitates uptake of the nucleosides through nucleoside receptors into the cell, where they can again be phosphorylated to generate nucleotides and contribute to the nucleotide pool, inside the cell. 5′-NT levels are elevated in hepatic diseases such as viral hepatitis, alcoholic liver disease and cirrhosis. |
| Features and Benefits【特点和优势】 | 5′-Nucleotidase (CD73) Activity Assay Kit:
|
| Principle【原理】 | The 5′-Nucleotidase (CD73) Activity Assay Kit is a simple two-step end point assay that relies on the Berthelot′s test for quantification of ammonia. In this assay, the action of 5′-nucleotidase on the substrate generates a product, which releases ammonia in presence of the converter. Developer I and II are then used to quantify the released ammonia through increase in absorbance at A670 nm. This assay can detect as low as 0.2 mU of 5′-NT. Since non-specific enzymes like alkaline phosphatase can give a positive signal in this assay, 5′-NT inhibitor may be used to completely inhibit 5′-nucleotidase and distinguish from the signal from non-specific enzymes. The assay kit also includes 5′- Nucleotidase (5′-NT) enzyme for use as a positive control. |
安全信息
| Pictograms【象形图】 | GHS05,GHS08,GHS09 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H317 - H318 - H334 - H341 - H350i - H360D - H372 - H373 - H411 |
| Precautionary Statements | P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313 |
| Hazard Classifications【危险分类】 | Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation |
| Target Organs【靶器官】 | Lungs |
| Storage Class Code【储存分类代码】 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
