Thioredoxin Reductase Kit

100

Thioredoxin Reductase Kit|sigma-aldrich

colorimetric

−20℃

The kit is suitable for the measurement of thioredoxin reductase (TrxR) in various samples including tissue, cells and serum.

Thioredoxin reductase (TrxR) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.

Thioredoxin Reductase Kit:

• Simple and fast procedure with results in ∼40 minutes.

In the assay TrxR catalyzes the reduction of 5, 5′-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generates a strong yellow color at A₄₁₂ nm. In crude biological samples, other enzymes such as glutathione reductase and glutathione peroxidase can also reduce DTNB. Therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed. The first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

GHS05

Danger

H315 - H318

P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364

Eye Dam. 1 - Skin Irrit. 2

10 - Combustible liquids