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Human CRISPR Inhibition Dolcetto Library
产品性质
packaging【包装】 | pkg of 10 vials (5x200µL aliquots of each component) |
concentration【浓度】 | ≥5x108 VP/ml (via p24 Assay) |
application(s) | CRISPR |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
基本信息
General description【一般描述】 | The Human CRISPR inhibition (CRISPRi) library (Dolcetto) is a single compact library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening using a KRAB-dCas9 effector. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018). Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative. |
Application【应用】 |
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Features and Benefits【特点和优势】 |
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Components【组分】 | 2 Subpools with a minimum concentration of 5x108 VP/mL(via p24 assay) PCRISPR003A - Human CRISPR Inhibition Dolcetto Library Set A PCRISPR003B - Human CRISPR Inhibition Dolcetto Library Set B |
Principle【原理】 | The power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease. CRISPR is most commonly used to create double-stranded breaks that often result in loss of gene function (CRISPR-KO). However, the full extent of CRISPR′s utility extends beyond just targeted cutting of DNA. CRISPR′s Nuclease-independent applications provide equal targeting specificity. Instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown complements CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi. |
Legal Information【法律信息】 | LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES |
安全信息
Pictograms【象形图】 | GHS07 |
Signal word【警示用语:】 | Warning |
Hazard Statements | H317 |
Precautionary Statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
Hazard Classifications【危险分类】 | Skin Sens. 1 |
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |