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LCFC-LCAT 脂肪酰转移酶活性检测试剂盒
LCFC-LCAT Acyltransferase Activity Assay
产品编号: | 4072367 |
规格: | Supplied by Roar Biomedical, Inc., sufficient for 100 fluorometric tests |
包装规格: | 1 KIT |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
LCFC-LCAT 脂肪酰转移酶活性检测试剂盒
LCFC-LCAT Acyltransferase Activity Assay
基本信息
NACRES | NA.84 |
General description【一般描述】 | The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol forming cholesteryl ester. This activity occurs on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT are incorporated into the core of HDL. |
Features and Benefits【特点和优势】 |
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Principle【原理】 | The LCFC-LCAT Acyltransferase Activity Assay is a fluorometric assay useful for measuring the acyltransferase activity of LCAT in serum or plasma. The method detects changes in LCAT free cholesterol (LCFC) levels in the sample without the use of cholesterol oxidase, peroxidase, or the generation of hydrogen peroxide. Detection is not affected by iodoacetate or other LCAT inhibitors. LCAT activity results in a fluorometric (λEx = 320 nm/λEm = 405 nm) product proportional to the amount of free cholesterol present. |
产品性质
usage【用途】 | sufficient for 100 fluorometric tests |
Detection Method | fluorometric |
storage temp.【储存温度】 | 2-8℃ |
suitability【适用性】 | Suitable for high-throughput screening, mechanism of action studies and structureactivity relationship (SAR) work of LCAT in plasma and serum |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |