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LDH-Cytotoxicity Assay Kit II
产品性质
| Quality Level【质量水平】 | 100 |
| web metadata keyword.default | LDH-Cytotoxicity Assay Kit II|sigma-aldrich |
| Detection Method | colorimetric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | The kit is suitable for the determination of Lactate dehydrogenase (LDH) activity in cells. |
基本信息
| General description【一般描述】 | Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study. |
| Features and Benefits【特点和优势】 | The LDH-Cytotoxicity Assay Kit II:
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| Principle【原理】 | The LDH Cytotoxicity Assay Kit II utilizes the advanced WST (Water Soluble Tetrazolium) reagent for a fast and more sensitive detection of LDH released from the damaged cells. The assay utilizes an enzymatic coupling reaction: LDH oxidizes lactate to generate NADH, which then reacts with WST to generate yellow color at A450 nm. The intensity of the generated color correlates directly with the cell number lysed. |
安全信息
| Pictograms【象形图】 | GHS05 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H314 |
| Precautionary Statements | P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| Hazard Classifications【危险分类】 | Eye Dam. 1 - Skin Corr. 1B |
| Storage Class Code【储存分类代码】 | 8A - Combustible, corrosive hazardous materials |
