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Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)
产品性质
Quality Level【质量水平】 | 100 |
web metadata keyword.default | Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)|sigma-aldrich |
Detection Method | colorimetric |
storage temp.【储存温度】 | −20℃ |
suitability【适用性】 | The kit is suitable for the measurement of PHGDH activity in various tissues (liver and kidney, etc.) and cells (adherent or suspension cells) and for the analysis of de novo L-serine biosynthesis. |
基本信息
General description【一般描述】 | Phosphoglycerate Dehydrogenase (PHGDH) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3 phosphohydroxyglycerate and the NADH-dependent reduction of a-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer. |
Features and Benefits【特点和优势】 | Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit:
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Principle【原理】 | The Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, phosphoglycerate dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal at A450 nm. |
安全信息
Pictograms【象形图】 | GHS05,GHS07 |
Signal word【警示用语:】 | Danger |
Hazard Statements | H314 - H335 |
Precautionary Statements | P261 - P271 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 |
Hazard Classifications【危险分类】 | Eye Dam. 1 - Skin Corr. 1B - STOT SE 3 |
Target Organs【靶器官】 | Respiratory system |
Storage Class Code【储存分类代码】 | 8A - Combustible, corrosive hazardous materials |