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Fire Monkey / Fire Flower High Molecular Weight-DNA Extraction Kit

产品编号:4073801
包装规格:1 kit
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40738011 kit3220
产品性质
feature【特点】
Includes Columns in 2 mL Collection Tubes, Lysis Solution DNA, Binding Solution, Wash Solution, Elution Buffer
packaging【包装】
kit of sample (10 extractions)
kit of 1 ea (50 extractions)
storage temp.【储存温度】
room temp
基本信息
General description【一般描述】
For the extraction of high molecular weight DNA from animal and bacterial cells, and size selection of extracted DNA from all sample types.

FIRE MONKEY - HMW DNA EXTRACTION
Fire Monkey is a standard spin-column process which extracts HMW-DNA with average strand lengths of 100kb and above from both bacterial and mammalian cells within 1hr.

FIRE FLOWER - DNA SIZE SELECTION KIT
Fire Flower is a standard spin-column process that can size select extracted DNA from all sample sources within 15mins. Fire Flower can increase the overall average strand length by 30%, while depleting DNA fragments up to 30kb. Fire Flower can be used to size-select up to 200 μL of extracted DNA despite the sample being rich in small DNA fragments.


The two major disruptive features of Fire Monkey are that firstly, it generates average DNA fragment lengths of between 100kbp – 130kbp and secondly, it significantly reduces the amount of small DNA fragments shorter than 10kbp in the final product. This combination, where significant amounts of the DNA fragments have intact lengths of over 165,000 base pairs and up to several hundred thousands base pairs in length, combined with the absence of most of the smaller DNA fragments, through its inbuilt size selection capability, massively improves the eventual long-read sequencing of the sample. Fire Monkey improves the N50 measure of sequencing quality by 100% and yet it takes a sixth of the time to do so compared to the main competing NAIP kits.
Application【应用】
Fire Monkey and Illumina reads used for AMR Characterization:
Rasheed F et al
Microorganisms 8 (9) 1336 Emergence of Resistance to Fluoroquinolones and ThirdGeneration Cephalosporins in Salmonella Typhi in Lahore, Pakistan

Fire Monkey and combined Nanopore/Illumina reads used for complete genome assembly of rare food chain pathogen. Public Health England reference lab:
Gao R et al
Microbiology Resource Announcements Volume: 9, Issue: 35. e0028020 Complete Genome Assemb lies of the Rare Salmonella enterica Serovar Adjame Using Nanopore and Illumina Sequence Reads

Fire Monkey and Nanopore reads used to characterize emerging resistance in a foodchain pathogen subpopulation:
Zhang CZ et al
Journal of Antimicrobial Chemotherapy dkaa288 Emergence of ciprofloxacin heteroresistance in foodborne Salmonella enterica serovar Agona.

Fire Monkey and 10xIllumina reads used for animal chromosome haplotyping. DanaFarber Cancer
Institute, Harvard University:
Tourdot RW & Zhang CZ
bioRxiv
Determination of complete chromosomal haplotypes by bulk DNA sequencing

Fire Monkey Total Nucleic Acid and PCR analysis of DNA and messenger RNA used to detect targets of
clinical infectious disease samples:
Todd AV & Lima NE
Nucleic Acid Ratio Determination (Patent Application). Pub. No.: US 2020/0199651
Features and Benefits【特点和优势】
  • Protocols are user-friendly and can be high-throughput.
  • Only basic laboratory equipment is required
  • Extract and purify long-fragments of DNA within one hour
  • In-built size-exclusion limits fragments <10kb
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H315 - H319
Precautionary Statements
P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
Hazard Classifications【危险分类】
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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