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Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
| 产品编号： | 4075599 |
| 规格： | affinity isolated antibody, buffered aqueous glycerol solution |
| 包装规格： | 0.25 ML，0.5 ML，1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD00162335 |
| NACRES | NA.46 |
| General description【一般描述】 | Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. |
| Immunogen【免疫原】 | purified goat IgG |
| Application【应用】 | Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate. Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) . |
| Biochem/physiol Actions【生化/生理作用】 | IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus, they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins. |
| Other Notes【其他说明】 | Antibody adsorbed with human serum proteins. |
| Physical form【外形】 | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide |
| Preparation Note【制备说明】 | Adsorbed to reduce background staining with human samples. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | alkaline phosphatase conjugate |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous glycerol solution |
| species reactivity | goat |
| technique(s) | direct ELISA: 1:30,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 western blot: 1:30,000 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
