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抗-人 IgM(μ-链特异性)-碱性磷酸酶 山羊抗
Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody produced in goat
产品编号: | 4075606 |
规格: | affinity isolated antibody, buffered aqueous glycerol solution |
包装规格: | 0.25 ML,0.5 ML,1 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
抗-人 IgM(μ-链特异性)-碱性磷酸酶 山羊抗
Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody produced in goat
基本信息
MDL number | MFCD00162460 |
NACRES | NA.46 |
General description【一般描述】 | Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. These immunoglobulins have been implicated in CNS myelin repair. Increased levels of secretory IgM have been linked to rhino-conjunctivitis . Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins. Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively. |
Immunogen【免疫原】 | Human IgM |
Application【应用】 | Goat polyclonal anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA. IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma). Goat Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody can be used for dot blot and western blot applications at 1:30,000. |
Physical form【外形】 | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
Legal Information【法律信息】 | CDP-Star is a registered trademark of Tropix, Inc. |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | goat |
conjugate【偶联物】 | alkaline phosphatase conjugate |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | secondary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous glycerol solution |
species reactivity | human |
technique(s) | direct ELISA: 1:30,000 dot blot: 1:30,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 western blot: 1:30,000 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |