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Procathepsin L human
| 产品编号： | 4075994 |
| 规格： | recombinant, expressed in E. coli, ≥90% (SDS-PAGE) |
| 包装规格： | 100 μG，1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Procathepsin L human
CTSL1
CTSL
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80℃. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution. |
| Physical form【外形】 | 1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥90% (SDS-PAGE) |
| form【形式】 | liquid |
| mol wt【分子量】 | 39.4 kDa (18-333 aa + N-terminal poly-his tag.) |
| packaging【包装】 | pkg of 1 mg pkg of 100 μg |
| concentration【浓度】 | 1 mg/mL |
| UniProt accession no.【UniProt登记号】 | P07711 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... CTSL1(1514) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
