Caspase-3 Substrate IV, Fluorogenic-CAS 1177935-21-5-Calbiochem

C₃₆H₃₈F₃N₅O₁₄

821.71

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.
Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Product does not compete with ATP.
Reversible: no
Primary Target
Fluorogenic caspase-3 substrate.
Cell permeable: no

Toxicity: Standard Handling (A)

Z-Asp-Glu-Val-Asp-AFC

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze

white to off-white

DMSO: 10 mg/mL

λ_ex ~400  nm
λ_em ~505 nm

ambient

−20℃

1, 5 mg in Plastic ampoule

11 - Combustible Solids

WGK 1