Cleland′s Reagent, ULTROL^® Grade

C₄H₁₀O₂S₂

154.25

MFCD00004877

A protective agent for SH groups.
Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

Toxicity: Harmful (C)

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.

Chau, M-H., and Nelson, J.W. 1991. FEBS Lett.291, 296.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry27, 3785.
Shaked, Z., et al. 1980. Biochemistry19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥99% (titration)

solid

Calbiochem^®

OK to freeze

white

water: 200 mM
ethanol: soluble

heavy metals: <1 ppm

2-8℃

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1

VHJLVAABSRFDPM-IMJSIDKUSA-N

GHS05,GHS07

Danger

H302 - H315 - H318

P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

11 - Combustible Solids

WGK 3