Propidium Iodide-CAS 25535-16-4-Calbiochem

C₂₇H₃₄I₂N₄

668.39

MFCD00011921

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M^-1cm^-1 (493 nm); 55,700 M^-1cm^-1 (287 nm); 13,800 M^-1cm^-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Irritant (B)

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

solid

Calbiochem^®

OK to freeze
protect from light

dark red

sample type intact cells

λ_ex ~536 nm
λ_em ~617 nm

ambient

2-8℃

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

XJMOSONTPMZWPB-UHFFFAOYSA-M

1 g in Plastic ampoule
250 mg in Plastic ampoule
50, 100 mg in Glass bottle

GHS08

Warning

H341

P201 - P202 - P280 - P308 + P313 - P405 - P501

Muta. 2

11 - Combustible Solids

WGK 3