CHAPS, Molecular Biology Grade-CAS 75621-03-3-Calbiochem

C₃₂H₅₈N₂O₇S

614.88

MFCD00012116

Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.
Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.
Conductivity (1.0 M, 24°C): <100 µmhos.

CHAPS 是一种用于膜生物化学的非变性两性离子洗涤剂。
可用于溶解膜蛋白以及打断蛋白-蛋白相互作用。CHAPS 具有低胶束分子量 (6,150) 和高临界胶束浓度 (6-10mM)，因此可通过透析从样品中除去。它也适合在等电聚焦和二维电泳中用于溶解蛋白质。CHAPS 常用于非变性(无尿素)等电聚焦，已发现它对某些亚细胞制备物和植物蛋白能够得出优异的分辨率。通常将介于 2-4% (w/v) 间的浓度用于等电聚焦凝胶。

Toxicity: Harmful (C)

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

Chen, Y., et al. 1992. Biochemistry 31, 2415.
Ofri, D., et al. 1992. J. Neurochem. 58, 628.
Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.
Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.
Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.
Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.
Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

100

≥98% (NMR)

crystalline powder

micellar avg mol wt 6150

Calbiochem^®

OK to freeze
desiccated (hygroscopic)

10

white

6 - 10 mM
6 mM (20-25℃)

cloud point >100 ℃

water: 1.0 M

DNase, RNase, and Protease, none detected

ambient

15-25℃

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

UMCMPZBLKLEWAF-RFCNGIAKSA-N

1 g in Glass bottle
25, 250 g in Glass bottle
1 kg in Glass bottle
5, 10 g in Plastic ampoule

11 - Combustible Solids

WGK 3