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Anti-Human IgG (γ-chain specific), F(ab′)2 fragment-Peroxidase antibody produced in goat
产品编号: | 4077206 |
规格: | affinity isolated antibody, buffered aqueous solution |
包装规格: | 1 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
MDL number | MFCD00162447 |
NACRES | NA.46 |
General description【一般描述】 | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific), (F(ab′)2) fragment-Peroxidase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins. |
Immunogen【免疫原】 | Purified human IgG |
Application【应用】 | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) Peroxidase-conjugated goat anti-human IgG (Fab specific) antibody was used for western blot and ELISA analysis of yeast cultures genetically modified to produced recombinant Fab fragments. Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene- diamine substrate solution (Sigma). Anti-Human IgG (γ-chain specific), (F(ab′)2) fragment-Peroxidase antibody is suitable for use in ELISA and western blot . |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative |
Preparation Note【制备说明】 | Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978). |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | goat |
conjugate【偶联物】 | peroxidase conjugate |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | secondary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
species reactivity | human |
technique(s) | direct ELISA: 1:10,000 western blot: suitable |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 1 mL in glass bottle |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Pictograms【象形图】 | GHS07,GHS09 |
Signal word【警示用语:】 | Warning |
Hazard Statements | H315 - H317 - H319 - H411 |
Precautionary Statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
Hazard Classifications【危险分类】 | Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 |
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |