Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

MFCD00162620

NA.46

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG.

Purified mouse IgG Fab fragment

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in:

• enzyme-linked immunosorbent assay (ELISA){47
• immunohistochemistry
• western blot

Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense.

Antibody adsorbed with human IgG.

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Adsorbed to reduce background staining with human samples.

200

goat

expressed in goat

alkaline phosphatase conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous solution

mouse

human

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

wet ice

2-8℃

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12 - Non Combustible Liquids

WGK 2