Cathepsin S, Human, Recombinant, E. coli

3.4.22.27 ( BRENDA | IUBMB )

Note: 1 mU = 1 milliunit.
Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase preferentially expressed in macrophages and microglia. Exhibits high specific activity.
Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.

Toxicity: Harmful (C)

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.

In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.

Following initial thaw, aliquot and freeze (-70°C).

Liuzzo, J.P., et al. 1999. Mol. Med.5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.
Kirschke, H., et al. 1989. Biochem. J.264, 467.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

expressed in E. coli

≥90% (SDS-PAGE)

liquid

≥30,000 mU/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

25 μg in Plastic ampoule

GHS07,GHS08

Warning

H302 - H373

P260 - P264 - P270 - P301 + P312 - P314 - P501

Acute Tox. 4 Oral - STOT RE 2 Oral

Kidney

10 - Combustible liquids

WGK 1