TO14

human ovary

Adherent

For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.

Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres.

Oestrogen receptor negative cell line.

Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.

cell culture | mammalian: suitable

metastasis

dry ice

Human ovarian cancer; oestrogen receptor negative, drug efficacy and toxicity

TO14 is an adherent cell line derived from a metastasis to the omentum obtained at hysterectomy from a patient with a well differentiated serous adenocarcinoma. TO14 exhibits poor growth in semi-solid medium (agar). TO14 is from the same patient as PEO23 and PEO14 cell lines (Sigma catalog numbers 10032313 and 10032311 respectively).

This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) available at ECACC which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists.

Not specified

RPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10⁴ cells/cm^2<sup/>

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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.