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产品性质
| biological source【生物来源】 | human blood |
| ethnicity【种族】 | French Canadian |
| gender【性别】 | female |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Lymphoblastoid |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
基本信息
| General description【一般描述】 | Homozygous: No Consanguineous: No |
| Cell Line Description【细胞系描述】 | This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line. |
| DNA Profile【DNA图谱分析】 | A: 03 25 B: 47 37 HLA DRB1: 0101 1417 HLA DRB3: 0101 HLA DQA1: 0101 0103 HLA DQB1: 0501 0603 HLA DPA1: 1 HLA DPB1: 401 |
| Culture Medium【培养基】 | RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%. |
| Subculture Routine【传代培养常规】 | Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium. |
| Other Notes【其他说明】 | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
产品说明
| Serological Profile【血清学分析】 | HLA A: 25 3 HLA C: 6 HLA B: 37 47 HLA DR: 1 6 HLA DR52: 52 HLA DQ: 1 |
