PITOUT human cell line

human blood

South African Caucasian

male

Suspension

Not specified

Lymphoblastoid

cell culture | mammalian: suitable

dry ice

Homozygous: Yes
Consanguineous: Yes

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

A: 2902
Cw: 1601
B: 44031
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 101
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 201
HLA DPA1: 1
HLA DPB1: 401

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

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HLA A: 29
HLA A ief: 29.2
HLA B: 44
HLA B ief: 44.1
Bw4/bw6: 4
HLA Dw: 17
HLA DR: 7
HLA DR53: 53
HLA DQ: 2
HLA DP: 4

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.