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Mitomycin C ready made solution
| 产品编号： | 4080006 |
| 规格： | 10 mg/mL in DMSO, ≥98% (HPLC), Cell culture tested |
| 包装规格： | 0.2 ML，5 X 0.2 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Mitomycin C ready made solution
MitoExtra
Mitonco
Mitocin C
Mutamycin
Mitozytrex
Ametycine
MMC
Mitoplus
Mitomycin
产品性质
| Quality Level【质量水平】 | 200 |
| Assay【测定】 | ≥98% (HPLC) |
| form【形式】 | liquid |
| storage condition【储存条件】 | protect from light |
| concentration【浓度】 | 10 mg/mL in DMSO |
| storage temp.【储存温度】 | −20℃ |
基本信息
| General description【一般描述】 | Mitomycin C (MMC) produced by Streptomyces caespitosus is an anticancer antibiotic agent which inhibits proliferation of various cell types in vitro. MMC is DNA alkylating agent, that causes cross linking of complementary DNA strands resulting in: mutagenesis, inhibition of DNA synthesis, initiation of DNA repair events, and activation of apoptosis. Furthermore, MMC has an effect on ribosomal RNA leading to protein synthesis inhibition. MMC is capable of inhibiting cell proliferation of human or murine embryonic fibroblast cells at concentration range of 0.2-20μg/mL. These treated cells are subsequently used as an adherent feeder layer in long-term culture assays mainly for growth of pluripotent stem cells. Feeder layer cells support the target cells by releasing growth factors to the cell culture and detoxification of the medium. Moreover, feeder layer cells play a role in ECM proteins synthesis that controls cell culture growth. In addition, feeder layer cells are used as a substrate for attachment of the target cells. MMC also was used for BLC (basal-like cancer) cell line. MMC as antibiotic agent showed a potent activity against MDR gram-negative bacteria like P. aeruginosa (2 μg/mL), E.Coli (0.5μg/mL) and A. baumannii (16μg/mL). MMC also has a strong activity against gram positive bacteria (0.2μg/mL). Technical information 1.Mitomycin C ready made solution provides a safer alternative to the powder form by preventing hazardous particles exposure. 2.Please avoid repeated freeze thaw cycles of this product. 3.Mitomycin C solutions are light sensitive and should kept in the dark. 4.It is recommended to keep Mitomycin C solutions at pH 6-7. 5.The recommended concentration of Mitomycin C in cell culture assays is 0.2-20μg/mL. (Ref.8,9,12,13) Therefore, it is recommended to dilute the ready made solution 1:500-50,000. |
| Caution【注意】 | Light sensitive |
| Physical form【外形】 | Liquid - In DMSO |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
| Flash Point(F)【闪点(F)】 | 188.6 °F - closed cup |
| Flash Point(C)【闪点(C)】 | 87 ℃ - closed cup |
