KAPA2G 耐用热启动 ReadyMix

NA.55

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.
KAPA2G Robust HotStart^® ReadyMix^™ is ideally suited for the amplification of DNA fragments.

KAPA2G Robust HotStart^® ReadyMix^™ has been used in:

• PCR
• Real time RT-PCR
• Nested Polymerase Chain Reaction (nPCR)
• Amplification of GC- or AT-rich templates
• Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
• Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
• Colony PCR

The KAPA2G Robust HotStart ReadyMix may be combined with KAPA Express Extract for fast and efficient DNA extraction and PCR. For more information, refer to the application notes for:

• Blood PCR
• Mammalian DNA barcoding
• Fish DNA barcoding
• FFPE PCR

KAPA2G Robust HotStart^® DNA Polymerase generated DNA fragments have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase, and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 10⁵ nucleotides incorporated. Like wild-type Taq, KAPA2G Robust HotStart has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity.

Each batch of KAPA2G Robust HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Robust HotStart ReadyMix PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4℃ is not recommended. Please note that reagents stored at temperatures above -20℃ are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC

组份不可单独销售
KAPA2G Robust HotStart^® DNA Polymerase 1U per 25 µL reaction
MgCl₂ 2mM at 1X
dNTP Mix 0.2 mM of each dNTP at 1X

100

sufficient for 100 reactions
sufficient for 500 reactions

≤12 mo.

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

kit of 1.25 mL (100 x 25 μL rxn; KK5704)
kit of 6.25 mL (500 x 25 μL rxn; KK5705)

Roche

2 ×

PCR: suitable

crude DNA

−20℃

GHS08

Warning

H371

P260 - P264 - P270 - P308 + P311 - P405 - P501

STOT SE 2

12 - Non Combustible Liquids

WGK 1